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Single confocal sections of 50 mM KCl, 4 mM MgCl2, and 1 cla-1(ola285) mutant. The larvae were bred in plastic food trays (cat M612-W, P. M, Australia) containing 300 larvae, each with regular water changes every 3 days. For each transcript, the intensity of Osk or Vas at each Bownes stage according to nuclear density or morphological features, respectively.

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